A-082 Measuring Peroxynitrite as an Early Biomarker for Cystic Fibrosis Using Organoselenide-Modified Glassy Carbon Electrode

Abstract Background Cystic fibrosis (CF) is a genetic disease that affects the respiratory, digestive, and reproductive systems. One of issues associated with CF oxidative stress, which an imbalance between production reactive oxygen species (ROS) ability body to neutralize them. Peroxynitrite, member ROS, very noxious molecule formed from reaction nitric oxide (NO) superoxide can damage cellular components such as proteins, lipids, DNA. Studies have shown peroxynitrite levels are elevated in people this increased stress contribute development lung damage, airway obstruction, other symptoms disease. Therefore, understanding role pathogenesis important for developing new therapeutic strategies reduce improve quality life Methods In study, we quantified levels, could serve early biomarker CF. A modified glassy carbon electrode (GCE) series organoselenide compounds was adopted using electro-grafting method measure situ. Scanning electron microscopy (SEM) energy-dispersive X-ray spectroscopy (EDX) were used examine structure surface chemistry organoselenide. Quartz Crystal Microbalance (QCM) analysis determine thickness electrodeposited selenium on GCE surface. Results Our organoslenide allowed application low positive potential, enhances separation analytes sensitive higher potentials. Cyclic voltammetry amperometry showed sensitivity at 110 nA/mol, detection limit recorded 10 micromolar. This electrochemical technique our electrodes be miniaturized detect cell line. The probe, constructed, utilized target ratiometric changes several slices, healthy inflammation cystic fibrosis, via fluctuation. addition, alterations prevalence strong linear association, resulting good potential predicting progression stage improving effective treatment. Conclusion study aimed quantify patients We levels. micromolar, results suggest peroxynitrite’s sensors by its pathogenesis,